Effect of salicylic acid on the activities of phenylammonialase (PAL), tyrosine ammonialase (TAL) and peroxidase (POD) on potato plant under in vitro culture

Maleki, Mohammad Sadegh

doi:10.22034/jpr.2025.8455.3355

Effect of salicylic acid on the activities of phenylammonialase (PAL), tyrosine ammonialase (TAL) and peroxidase (POD) on potato plant under in vitro culture

Articles in Press

Document Type : Research Paper

Author

Mohammad Sadegh Maleki

Dept of Plant and Animal Science, Faculty of Biological Science and Technology

10.22034/jpr.2025.8455.3355

Abstract

Potato is one of the crop plants can be used as a model in biological studies. Salicylic acid as a stimulator induces growth by producing reactive oxygen species. This hormone can increase the antioxidant capacity and secondary metabolites accumulation such as phenol, flavonoid, anthocyanin and enzymes involved in the biosynthetic pathway of these compounds. In this study, potato seedlings were grown in MS medium under concentrations of 0, 0.1, 1, 10, and 100 μM salicylic acid. The results of this research showed that salicylic acid in low concentrations (0.1, 1 and 10 μM) increased the fresh and dry weight of the plant compared to the control samples. Also, the content of phenolic, flavonoid and anthocyanin compounds increased with increasing concentration of salicylic acid. In addition, salicylic acid improved the activity of phenylammonialyase, tyrosine ammonialyase, peroxidase and antioxidant capacity, the highest results were observed at the concentration of 100 μM. Based on these results, it can be suggested that due to the inductive roles of salicylic acid, the growth indicators and some secondary metabolites in potato plants increased under in vitro conditions.

Keywords

Phenolic compounds

Salicylic acid

In vitro culture

Potato

Reactive oxygen species

Subjects