Effects of Percidine, Aqua Percidine, Percidine Plus and Super Percidine on reducing contamination from culture medium used in Buxus hyrcana Pojark tissue culture

Document Type : Research Paper


Assistant Prof. Research Division of Natural Resources, Golestan. Agriculture and Natural Resources Research and Education Center, AREEO, Gorgan, Iran


Buxus hyrcana Pojark is one of the endangered trees in the Hyrcanian forests of Iran, which has been faced by pests and diseases in recent years. Due to the importance of this species and the need to its tissue culture, finding a suitable method for sterilization of explants of this forest species is essential. Therefore, the aim of this study was to investigate the effects of Percidine, Aqua Percidine, Percidine Plus and Super Percidine in reducing pollution from the culture medium in forest boxwood tissue culture contamination from culture medium used in Buxus hyrcana tissue culture. The experiment was performed in a completely randomized design with 3 replications (n=45). First, for pre-treatment, explants (twigs and seeds) were washed with ordinary water and a few drops of dishwashing liquid for 5 minutes to remove surface contaminants. Then, they were placed in benomyl fungicide (1%) for 10 minutes. After washing, under sterile hood, sterilization of explants was performed through usual method (using ethanol 96% and sodium hypochlorite (1:3 mixed with water) and washing with distilled water three times for 10 minutes). Experimental treatments included disinfectant (Percidine 15%, Aqua Percidine, Super Percidine, Percidine Plus (at concentrations of 200, 400 and 600 μl) in combination with the culture medium. After sterilization of explants, plant materials were cultured on Murashige and Skoog media in combination with disinfectants. To measure contamination, cultures were examined under growing conditions at 25± 1 °C with a light intensity of 1000 to 1500 lux, 16 hours of light


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Articles in Press, Accepted Manuscript
Available Online from 10 January 2023
  • Receive Date: 06 September 2022
  • Revise Date: 18 December 2022
  • Accept Date: 03 January 2023