Document Type : Research Paper

Authors

• mohsen bagherian ¹
• Esmaeil Chamani ²

¹ PHD student

² Dean of Agriculture Faculty

Abstract

Lilium ledebeourii (Baker) Boiss is one of the most unique ornamental species, and the breeding program of this plant is very important. As this flower is a wild species, breeding efforts are rare. The goal of this study was to investigate the effects of different plant growth regulator combinations on the embryogenesis and regeneration of Lilium ledebeourii (baker) Boiss by ovary culture. This research was carried out as a factorial arrangement based on a completely randomized design with 5 replications. The treatments for callus production was 0.5 mg/l BA plus 0.5mg/l NAA and various concentrations of picloram (0,0.5,1,2,mg/l) and tidiazoron (0,0.5,1,2,mg/l) were used for embryogenesis. The B5 medium without hormones was used for induction of regeneration. The measured traits in this research included the embryo number, embryogenesis percentage, embryo diameter, embryo length, embryo fresh weight and callus fresh weight. The results showed that the interaction effect of picloram and tidiazoron significantly (P<0.05) affected the number and diameter of embryos. The callus weight was significant at a 1% probability level. However, the main effect of thidiazuron and picloram was significant on embryo weight and percentage of embryogenesis respectively. The highest amount of embryo fresh weight and embryo diameter (4.06 mm) was obtained in a medium containing 1 mg/l TDZ and 0.5 mg/l TDZ+1 mg/l Pic, respectively. The highest (86.35%) percentage of rooting in regenerated plantlets was observed in the B5 medium containing 0.5 mg/l of TDZ.

Keywords

• Callus
• Embryogenesis
• Lilium ledebeourii
• Ovary
• Picloram

Main Subjects

• Tissue culture