TY - JOUR ID - 2199 TI - Investigation on embryogenesis of Lilium ledebeourii Boiss by ovary culture under in vitro condition JO - Journal of Plant Research (Iranian Journal of Biology) JA - JPR LA - en SN - 2383-2592 AU - bagherian, mohsen AU - Chamani, Esmaeil AD - PHD student AD - Dean of Agriculture Faculty Y1 - 2022 PY - 2022 VL - IS - SP - EP - KW - Callus KW - Embryogenesis KW - Lilium ledebeourii KW - ovary KW - Picloram DO - N2 - Lilium ledebeourii (Baker) Boiss is one of the most unique ornamental species, and the breeding program of this plant is very important. As this flower is a wild species, breeding efforts are rare. The goal of this study was to investigate the effects of different plant growth regulator combinations on the embryogenesis and regeneration of Lilium ledebeourii (baker) Boiss by ovary culture. This research was carried out as a factorial arrangement based on a completely randomized design with 5 replications. The treatments for callus production was 0.5 mg/l BA plus 0.5mg/l NAA and various concentrations of picloram (0,0.5,1,2,mg/l) and tidiazoron (0,0.5,1,2,mg/l) were used for embryogenesis. The B5 medium without hormones was used for induction of regeneration. The measured traits in this research included the embryo number, embryogenesis percentage, embryo diameter, embryo length, embryo fresh weight and callus fresh weight. The results showed that the interaction effect of picloram and tidiazoron significantly (P<0.05) affected the number and diameter of embryos. The callus weight was significant at a 1% probability level. However, the main effect of thidiazuron and picloram was significant on embryo weight and percentage of embryogenesis respectively. The highest amount of embryo fresh weight and embryo diameter (4.06 mm) was obtained in a medium containing 1 mg/l TDZ and 0.5 mg/l TDZ+1 mg/l Pic, respectively. The highest (86.35%) percentage of rooting in regenerated plantlets was observed in the B5 medium containing 0.5 mg/l of TDZ. UR - https://plant.ijbio.ir/article_2199.html L1 - ER -