Chuchaq (Eryngium caucasicum), is a perennial plant belonging to the Apiaceae, it has various medicinal properties such as analgesic, appetizing, diuretic, anti-inflammatory and antioxidant effects. The aim of this study was to investigate the effect of the combination of growth regulators on callus production, shoot induction, rhizogenesis and proliferation of Chuchaq in vitro condition. Young and healthy leaves and petioles were used as explants and were cultured in MS, MS1/2 and MS 1/4 with different concentrations of (BAP, NAA, KIN, IAA). In MS medium with 1BAP + 0.1NAA mgL-1, the most callus induction and regeneration were performed. Fifteen days after placing the explants in the culture medium, white calli were formed. After 21 days, the first signs of the formation of new shoots were revealed. The emerging shoots with the height of 2-2.5 cm were transferred to MS medium for root induction, almost all were rooted in MS without growth regulators. Along with the formation of roots, they were formed propagules from the collar area of the plantlets. The plantlets were taken out of invitro condition and were transplanted into sterilized mixtures of a coco peat and Perlit, and to gradually reduce the humidity, they were kept in the greenhouse under special conditions. In MS1/2 and MS1/4 media, callus formation and processing were reported as favorable, however in final adaptation of plants to soil conditions, 70% of regenerated plants in culture medium (1BAP + 0.1NAA) mgL-1, adapted well to the soil and grew well in pots.