Response of nutrient accumulation and antioxidant enzymes gene expression of fennel Foeniculum vulgare Mill.) to nano and bulk NiO2

Document Type : Research Paper

Authors

1 1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

3 2Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Abstract

Extensive and unlimited use of nanoparticles has attracted the attention of researchers to study their environmental effects. In this study, the effects of nano and bulk nickel oxide treatments on nutrients accumulation and gene expression of fennel were investigated. Nanoparticle sizer was used to measure particle size and zeta potential, XRD for the crystal structure and AFM for particle size. After planting the plants in greenhouse conditions and treatment with nano and bulk NiO, the content of different nutrients in the roots and leaves of the plant and antioxidant enzymes gene expression by Real-Time PCR, were investigated. The results showed that in the control the highest accumulation of elements occurred and tha applied treatments, especially 20 ppm nano,did not prevent the movement of elements to the leaf and at this concentration the displacement of elements except Ca and Na to the leaf was significant. Bulk NiO showed a greater decreasing effect on element uptake. Increased expression of ndhH gene was observed in all nano and bulk concentrations in comparison with the control and the highest increase was related to 800 ppm. In relation to ndhD gene, a decrease in gene expression was observed in different concentrations of nano and bulk NiO compared to the control, which is probably due to the involvement of other detoxification pathways against stress. According to the mentioned cases, it seems that fennel plant is resistant to nano and bulk NiO.

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Articles in Press, Accepted Manuscript
Available Online from 22 October 2022
  • Receive Date: 07 October 2021
  • Revise Date: 13 January 2022
  • Accept Date: 30 March 2022