Antibacterial and cytotoxicity effect of (Ficus carica) against HepG2 cells

Document Type : Research Paper

Authors

1 lantern research group. Karaj.iran

2 Young Research and Elite Club, Islamic Azad University, Saveh, Iran.

3 Department of Plant Science, Faculty of Sciences, Bu-Ali Sina University, Hamedan, Iran

Abstract

Nowadays, medicinal plants have the potential to discover new drugs. Liver cancer is known as one of the most common cancers in the world. F. carica is known as one of the most important medicinal plants around the world. In the present study, the antimicrobial and anticancer effects of F. carica were measured at different concentrations. In the antimicrobial assay, all bacterial strains, including Staphylococcus aureus, Ecoli, and Pseudomonas aeruginosa, were sensitive to the methanolic concentration of the extract. The MIC value was 8 µg/mL to 16 µg/mL and the MBC value was 16 µg/mL to 32 µg/mL. Hydroalcoholic extract of fig fruit with different concentrations (0.5, 0.75, 1, 1.5 mg/mL) on the growth of HepG2 liver cancer cells and L929 fibroblasts as a control (24, 48 and 72h) were assessed by MTT test. The results showed that the effect of F. carica extract is dose and time-dependent and with increasing time the bioavailability of cells has decreased and at the time of incubation for 72 hours and a concentration of 1.5 mg/mL bioavailability of cells was observed to be 8%. The results demonstrated that IC50 was observed in 0.5 mg/mL (24h). According to the results, it can be claimed that F. carica extract in different concentrations has an inhibitory effect on the growth of bacteria and liver cancer cells, so with further studies, this plant can be used in treatment.

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Articles in Press, Accepted Manuscript
Available Online from 28 August 2021
  • Receive Date: 19 November 2020
  • Revise Date: 11 April 2021
  • Accept Date: 24 June 2021
  • First Publish Date: 28 August 2021