Biochemical responses of Plargonium graveolens L′HER under UV-B radiation in In Vitro condition

Document Type : Research Paper


1 Department of Biology, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran.

2 Department of Biology< Faculty of Biological Sciences, Islamic Azad University, North Tehran Branch

3 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.


Pelargonium graveolens L ′Her is an aromatic and medicinal plant which is widely used in pharmaceutical, perfumery and food industry. In this study, the effect of ultraviolet radiation with different intensities (0, 0.12, 0.26 and 0.38 W/m2) was investigated on P. graveolens in InVitro condition. The amount of total phenol, flavonoid and anthocyanin, activity of phenylalanine ammonia lyase (PAL), photosynthetic pigments, carbohydrates, proteins and activity of some anti-oxidant enzymes (Catalase, peroxidase and superoxide dismutase) were evaluated. The antioxidant activity of methanolic extract was measured by using 2-2-di-phenyl-1-picaril hydrazil (DPPH) method. Total protein content and chlorophylls a, b showed a significant decrease (P≤ 0.05), while the amount of carotenoid increased significantly (P≤ 0.05), when ultraviolet radiation increased. With increasing UV-B radiation, the activity of studied enzymes, total phenols, flavonoids and anthocyanins content increased significantly (P≤ 0.05). Also, with increasing radiation intensity, the mean IC50 of methanolic extract decreased. The decreasing pattern of IC50 was consistent with the trend of increasing the mean of phenols. The present results show that P.graveolens in response to UVB stress has increased its antioxidant capacity by increasing the activity of antioxidant enzymes and increasing the phenols.


Main Subjects

Volume 35, Issue 1
April 2022
Pages 55-68
  • Receive Date: 05 June 2019
  • Revise Date: 23 October 2020
  • Accept Date: 30 November 2020
  • First Publish Date: 13 December 2020