Genetic diversity analysis in the local treasury of sugarcane germplasm in Khuzestan province using RAPD marker

Ahlam, Nabhani,; Soltani Howyzeh, Mehdi; Parvizi Almani, Masoud

doi:10.22034/jpr.2024.2357

Genetic diversity analysis in the local treasury of sugarcane germplasm in Khuzestan province using RAPD marker

Document Type : Research Paper

Authors

Nabhani, Ahlam ¹

Mehdi Soltani Howyzeh ¹

Masoud Parvizi Almani ²

¹ Department of Genetics and Plant Breeding, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.

² Research and training institute for the development of sugarcane

10.22034/jpr.2024.2357

Abstract

This study was performed to analyze the genetic diversity of sugarcane germplasm in Khuzestan province using the RAPD marker at Islamic Azad University, Ahvaz Branch, Iran. The sample size was 48 genotypes from the sugarcane germplasm of Khuzestan province. DNA extraction from leaves was performed using the potassium acetate SDS method. DNA quality test was done with electrophoresis (Agarose gel 1%) and DNA quantity was determined by spectrophotometer (Model: UV-Vis Camspec M350). Sixteen primers were selected for PCR and statistical analysis was performed by the NTSYS software. The Jaccard coefficient was used to determine the similarity of populations and the similarity matrix obtained by the UPGMA algorithm. After PCR, 54 fragments were amplified; among them, 53 bands (98%) were polymorph. OPM16 showed the highest band number (5 bands) and OPM36, OPM24 and OPA02 showed 4 bands. All primers showed 100% polymorphism except OPM27. According to cluster analysis, genotypes are divided into 4 groups. Three groups included a genotype and one group included 45 genotypes.

Keywords

Germplasm

Polymorphism

Genotype

PCR

Subjects