Document Type : Research Paper

Authors

• motahare omidipour ¹
• fatemeh jamal omidi ²

¹ Department of, Biology Faculty of Science, University of Guilan, Rasht, Iran

² Department of Biology, Faculty of Basic Sciences, University of Guilan, Rasht, Iran

Abstract

Gojiberry is considered one of the most important medicinal plants and has been called a super food or super fruit due to its high therapeutic effects. Considering the medicinal and edible importance of this species, its reproduction through tissue culture at a low cost over a large area is necessary. For this purpose, an experiment was conducted in the form of a completely randomized design with three replicates. First, the seeds were disinfected and planted in 1.2 MS medium, then in full MS culture medium containing different concentrations of hormones for branching ((2,3,0) BA, (0,5) IBA) mg/L, and callus formation was transferred with hormonal ratios (0.5, BA, (2.0, 2.4 D) mg/L individually and in combination. Each of the produced shoots was transferred to a medium containing hormones ((2,0) BA, (0,5) NAA) with and without activated charcoal to check the rooting power. According to the obtained results, the medium containing hormones (BAP: IBA) at a ratio of (0.3:5) has the highest average number of branches with an average of 2.99. The use of activated charcoal in the hormonal ratio (0.5:0) in the absence of BA caused potential stimulation of root growth. The investigation of secondary metabolites of phenol, flavonoid, and flavone showed that root, stem, leaf, and callus samples had different responses to different ratios of growth regulators.

Keywords

• Key words: Goji berry
• callus formation
• root formation
• activated charcoal
• secondary metabolite

Main Subjects

• Tissue culture