Document Type : Research Paper

Authors

• Hassan Sarikhani-khorami ¹
• حسن Sarikhani ²
• Abdolkarim Chehregani Rad ³

¹ 1- Department of Horticultural Science, Bu-Ali Sina University, Hamedan, Iran

² Department of Horticultural Sciences, Bu-Ali Sina University, Hamedan, Iran

³ Department of Biology, Bu-Ali Sina University, Hamedan, Iran.

Abstract

The use of vegetative rootstocks is essential for standardizing production and addressing challenges arising from seedling rootstocks in modern horticulture. This study aimed to establish an optimized protocol for disinfection, proliferation, and rooting of Myrobalan 29C, GF677, and Garnem rootstocks. Additionally, we investigated the potential impact of multi-walled carbon nanotubes (MWCNs) on the establishment and growth of these plants. DKW, WPM, and MS culture media were prepared, and single nodule explants were utilized to explore the influence of culture medium type and plant growth regulators. Among the three rootstocks, Myrobalan, Garnem, and GF677 explants exhibited the most favorable establishment in DKW, MS, and MS culture media, respectively. Notably, GF677 rootstock showed the highest number of branches, nodes, and branch length during the proliferation stage with a concentration of 1 mg/L of BA and 0.1 mg/L of NAA. Similarly, Garnem displayed the highest number of branches with a treatment of 2 mg/L of BA along with 0.25 mg/L of NAA. Regarding rooting, the highest rooting rate was achieved with Myrobalan, Garnem, and GF677 at concentrations of 1, 1, and 2 mg/L of IBA, respectively. Furthermore, the application of 50 mg/L of MWCNs resulted in increased explant growth; however, higher concentrations exhibited adverse effects on explant growth and development. The use of MWCNs holds potential to enhance tissue culture efficiency, particularly for explants of woody plants.

Keywords

• Stone fruit"
• rootstock"
• micropropagation"
• "
• carbon nanotubes"

Main Subjects

• Tissue culture