Document Type : Research Paper

Authors

• Vali Farzifard-Kambelash ¹
• Ahmad Moieni ²
• Raheleh Karimi-Ashtiyani ³
• Mohammad Sadegh Sabet ²

¹ Tarbiay Modares University

² Department of Plant Genetics and Breeding, College of Agriculture, Tarbiat Modares University, Tehran, Iran

³ Department of Plant Biotechnology, College of Agriculture, Tarbiat Modares University, Tehran, Iran

Abstract

Plant regeneration is one of the main stages of genetic manipulation, so that the production of transgenic plant is impossible without optimizing shoot regeneration protocol . In the present survey, the effects of the explant age (cotyledon) and hormone combination on shoot regeneration in Cucumis melo subsp. melo group inodorus var. Minoo 095p were investigated. For this purpose, decoated seeds were surface sterilized and cultured in half strength of MS medium. Two-, four- and eight-days old cotyledons were used for explant preparation. All cotyledonary explants were cultured in hormonal combinations of BAP (6-benzylaminopurine) with four levels (0, 0.25, 0.50, and 1.00 mg l-1) and IAA (indole-3-acetic acid) with two levels (0 and 0.01 mg l-1). The number of shoot primordia and shoots was evaluated after 6 weeks. The results showed that, the interaction of cotyledon age and hormone combination was significant on the number of shoot primordia and shoots . Besides, the mean comparison showed that, the combination treatment K3 (two-days old cotyledonary explants cultured in a hormone combination of 0.5 mg l-1 BAP and 0.01 mg l-1 IAA) was the most effective treatment for shoot primordium (6.2 primordia per explant) and shoot regeneration (3.87 shoots per explant). The obtained results can be used in the genetic manipulation programs of Persian melon.

Keywords

• plant regeneration
• cotyledonary explant
• shoot primordium
• 6-Benzylaminopurine
• and Indole-3-acetic acid

Main Subjects

• Tissue culture