Document Type : Research Paper

Authors

• Acram Ghochin ¹
• Marzieh Taghizadeh ²

¹ Dept of Plant and Animal Biology, Faculty of Biological Science and Technology,,University of Isfahan.

² Dept of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan.

Abstract

This study was aimed to investigate the effects of different concentrations of Ni (0, 5, 10, 25, 50, 75, 100 and 200 mg. l-1), on the antioxidant activity of two populations of serpentine and non-serpentine of Alyssum bracteatum and serpentine population of A. longistylum under in vitro culture. The uptake and accumulation of Ni in roots and shoots of tree population was determined. The results showed that, the accumulation of Ni in all populations increased with increasing of Ni concentration in MS medium. In all treatments, the amounts of Ni in the shoots were higher than roots. The amounts of Ni in shoots of both serpentine and non-serpentine were high, but the amounts of Ni in the roots of non-serpentine population were less than serpentine population. In all populations by increasing of Ni concentration in the medium the activity of catalase (CAT), ascorbate peroxidase (APX) and proline accumulation in shoots increased significantly compared to the control. Overall, the results showed that serpentine and non-serpentine populations of A. bracteatum and A. longistylum are able to accumulate Ni and considered as Ni hyperaccumulator plants

Keywords

• "
• Heavy metals"
• ,"
• Nickle"
• Plant tissue culture"
• , "
• Serpentine"

Main Subjects

• Physiology