Document Type : Research Paper
Authors
1 Bu-Ali Sina University
2 Assistant Professor Department of Agricultural Biotechnology Faculty of Agriculture Bu-Ali Sina University Hamedan, Iran
Abstract
Investigating the possibility of resistance to Fusarium Basal Rot in Azarshahr red onion cultivar using Melatonin
Abstract
An experiment was carried out using 0, 50, 100 and 300 μM melatonin in onion cultivar to investigate its effect on induction of resistance to Fusarium basal rot caused by Fusariumoxysporum f. sp. cepae. In this experiment, traits such as total phenolic content, hydrogen peroxide, malondialdehyde, antioxidant enzymes (catalase (CAT) and guaiacol peroxidase (GPX)) and disease-related enzymes (phenylalanine ammonia-lyase (PAL), chitinase and β-1,4-glucanase) were measured. The results showed an application of exogenous melatonin caused an increase in the total phenolic content and activity of antioxidants and disease-related enzymes, resulting a decrease malondialdehyde (MDA) and in the hydrogen peroxide (H2O2) level in Fusarium-treated plants. Although all concentrations of melatonin altered some biochemical and physiological traits in onions under Fusarium basal rot stress, the best overall result regarding induction of resistance to Fusariumoxysporum f. sp. cepae in these plants was observed on days 12 and 16 by applying 100 μM melatonin.
Keywords
Main Subjects