Document Type : Research Paper

Authors

• Nafiseh Najarian Kermani ¹
• Mahdieh Parsaeian ²
• Ziba Ghasimi Hagh ²

¹ Department of Agronomy and Plant Breeding, Shahrood University of Technology, Shahrood, I.R. of Iran.

² Assistant Prof, Shahrood University of Technology.

Abstract

The usual method of Haworthia attenuatae propagation hasn’t commercial returns due to the low number of offsets and rooting of leaf cuttings. In the present study, micro-propagation of this plant was evaluated using leaf explants and BAP, and KIN (0.5, 1 and 2 mg / l) in combination with NAA (0, 0.01, 0.1 and 0.5 mg/l). The results showed that the maximum number of shoots was regenerated using BAP in combination with NAA from the basal leaf segment explant. The maximum number of leaves (3.55) and the longest leaves (0.61 cm) were obtained on medium containing 2 mg/l BAP and 0.01 mg/l NAA using the basal leaf segment explant and the widest Leaves (0.44 to 0.48 cm) was observed on the medium containing 2 mg/l BAP without or with low concentration of NAA. The results of KIN application showed that the longest leaves (0.56 cm) with the maximum numbers (2.45) were obtained on medium containing 2 mg/l KIN and 0.01 mg/l NAA from the entire leaf explant. More over, the widest leaves (0.87 cm) was produced on medium supplemented with 2 mg/l KIN. The findings of rooting on MS and½ MS media supplemented with 0, 0.5, 1, 1.5 mg/l NAA showed that the highest number (4.33) and the longest roots (2.16 cm) were produced on ½MS medium containing 0.5 mg/l NAA. 90% of rooted plantlets were adapted to the greenhouse environment. Totally, the accurate optimization of this protocol can be applied to commercial micro-propagation of H. Attenuatae from leaf explants.

Keywords

• Micro-propagation
• Haworthia attenuate
• Growth regulator
• Leaf explant

Main Subjects

• Tissue culture