Transient expression of recombinant anti-VEGF protein based on viral vector (ZYMV) in Chenopodium album

Document Type : Research Paper


1 University of hormozgan

2 Department of Biotechnology, Tarbiat Modares University, Tehran, Iran

3 Department of Biotechnology and Plant Breeding, Ferdowsi University of Mashhad, Mashhad, Iran

4 Venom & Biotherapeutics Molecules Lab, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran


Transient expression through viral vectors has emerged as a preferred method for the production of plant recombinant protein. The short-term production of recombinant protein in this method, compared to time-consuming production of stable transgenic plants along with being cheaper and easier strategy pave the way for commercial production of recombinant proteins. In this research, Anti-Vascular endothelial growth factor (AntiVEGF) protein was produced in Chenopodium album plant using Zucchini yellow mosaic virus (ZYMV) virus-based transient expression method. Target plants were initially infected using a viral vector containing the GFP reporter gene (pZYMVGFP) to ensure the transgene expression. After the appearance of viral symptoms on plant leaves and also confirmation of plant infection by RT-PCR and dot blot methods, target gene cloning was performed in the pZYMVGFP virus vector. In the next step, the target plants were inoculated using a recombinant viral vector (pZYMVAntiVEGF) and the target gene expression was examined by RT-PCR at the transcription level and by Dot blot, Western blot and ELISA tests at translation level. Various molecular analysis confirm the recombinant AntiVEGF expression in this plant. Therefore, ZYMV viral-based transient expression system would be a suitable platform for recombinant proteins production in Chenopodium album plants.


Main Subjects

Volume 37, Issue 1
March 2024
Pages 77-92
  • Receive Date: 03 January 2022
  • Revise Date: 30 January 2022
  • Accept Date: 30 January 2022