The effect of EDTA on some physiological characteristics of Chenopodium botrys L. under Cd stress

Document Type : Research Paper

Author

Department of Plant Sciences, Faculty of Biological Sciences, Kharazmi University, Postal code 15719-14911, Tehran, Iran

Abstract

Cadmium (Cd) is one of the main soil contaminants. Applying EDTA to soil is a strategy to increase the heavy metal phytoremediation, but such chelators usually cause phytotoxicity and side effects. Therefore, the aim of this present study was to evaluate the effects of EDTA application on physiological and biochemical characteristics of Chenopodium botrys L. under Cd treatment in soil. In this study, the effect of EDTA (0, 3, 6 and 12 mmol kg-1) on the amount of cadmium absorbed by plant tissues, leaf relative water content, membrane stability index, photosynthetic pigments content, soluble carbohydrate content, total protein content and antioxidant enzyme activity and seed protein pattern of C. botrys were examined under Cd stress (0, 75, 150 and 225 mg kg-1). The results of this study showed that the addition of EDTA to the soil increased the concentration of Cd in shoot and roots of C. botrys. The application of EDTA reduced the leaf relative water content, membrane stability index and increased the photosynthetic pigments content and soluble carbohydrates content. Also changes in seed protein pattern, beside increase in total protein content and activity of antioxidant enzymes were observed in plants treated by Cd and EDTA, suggesting that this treatment was helpful in reducing of oxidative stress in C. botrys under Cd treatment. Based on the results, C. botrys is suitable for phytoremediation of soil contaminated with Cd. In addition, EDTA can play a significant role in removing this metal through transferring it from the soil to the plant.

Keywords

Main Subjects

Volume 35, Issue 2
June 2022
Pages 233-249
  • Receive Date: 21 January 2022
  • Revise Date: 20 February 2022
  • Accept Date: 04 March 2022
  • First Publish Date: 22 June 2022