Galegine is one of the most important active ingredients in the Galga (Galega officinalis L.), which is used as a drug for lowering blood sugar in the treatment of type 2 diabetes. The use of nano-elicitors to increase the production of secondary metabolites in plant cell and tissue culture is a useful method to achieve high levels of compounds with medicinal worth. In this study, the effects of nano-silver (5, 10, and 20 mg/L) stimuli, iron nano oxide (50, 100, and 200 mg/L), and molybdenum nanoparticles (30, 60, and 90 mg/L) on cell growth and production of some metabolites were studied in cell suspension culture of G. officinalis. The results showed that the application of silver nanoparticles in cell suspension culture at 5 and 10 mg/L for 48 h resulted in the highest galegine content. Also, the application of 100 mg/L iron nano oxide and 60 mg/L molybdenum led to an increase in galegine content in the cell suspension culture of G. officinalis compared with the control. The highest content of total phenols and flavonoids was obtained in the treatment of cells with 100 mg/L iron nano oxide. In general, considering that some treatments used increased the content of galegine in comparison with the control, it can be suggested that applying nano-elicitors in the cell suspension culture can be used to produce more galegine of this plant.