Zofai (Thymbra spicata L.) is a shrub plant belonging to the Lamiaceae family, with the local name Jaatenah, which has not been reported to grow in Lorestan province. This species is distributed in the range mountains of Darab Rika and Zaruni of Kouhdasht city. Zofai has long been used as a spice in traditional medicine to treat respiratory and gastrointestinal problems. This plant species has strong antimicrobial and antifungal properties due to its strong active ingredients. The samples were collected from the natural habitat and after identification and approval, they were dried at room temperature to provide essential oil. The water distillation method was used to extract the essential oil and ,and the chemical compounds of essential oil were characterized using Gas chromatography–mass spectrometry (GC-MS). According to the results of Zofai essential oil analysis, 25 compounds were identified that made up 97.04% of the total volume of essential oil. The major compounds identified in the essential oil of Zofai include Carvacrol (65.88%), γ-terpinene (9.71%), P-cymene (7.82%), Caryophyllene (2.51%), Myrcene (2.09%) , α-terpinene (2.04%). Phenolic compounds act as antimicrobials, antifungals, antioxidants and reduce inflammation. The presence of high Carvacrol in the essential oil of T. spicata, as a phenolic compound, confirms the traditional uses of the T. spicata medicinal species and this medicinal plant can be considered as a source of carvacrol production.