Effect of Urea on Nitrate Reductase Activity, Nitrate, Total Amino Acids and some Ions Concentration in Seedlings of Pistachio (Pistacia vera L.), under Sodium Chloride Stress

Document Type : Research Paper

Authors

1 Ramin University of Agriculture and Natural Resources, Mollasani, Khuzestan

2 Khuzestan Agricultural Sciences and Natural Resources University, Mollasani, Khuzestan, Iran

3 Department of Soil Science, Ramin University of Agriculture and Natural Resources, Mollasani

4 Department of Horticulture, Ramin University of Agriculture and Natural Resources, Mollasani, Khuzestan,

Abstract

In the present experiment, the effect of sodium chloride (0, 75 and 150 mM NaCl) and Urea (0, 60 and 120 mg/kg soil) on nitrate reductase activity (NRA) and accumulation of nitrate, total amino acids and some ions in the seedlings of pistachio (P. vera L. cv. ´´Badami Zarand´´) were studied. The results showed that application of 60 mg Urea per kg of soil in 0 and 75 mM sodium chloride treatments significantly increased the NRA in leaves (7.52 and 7.14 µ NO2-, respectively). In the seedlings treated with 75 mM sodium chloride, the total leaf amino acid content (AA) after application of Urea (120 mg/kg soil) increased significantly (22.91 µg. g FW), however, the AA in root of both salinity treatments (75 and 150 mM sodium chloride) was increased after application of 60 mg Urea per kg of soil. Application of 60 mg Urea per kg of soil resulted in a significant increase in the root/leaf ratio of the AA in 75 mM sodium chloride, but in 150 mM sodium chloride treatment, application of 120 mg Urea per kg of soil increased this proportion. Also, application of urea had a significant effect on nitrate accumulation in leaves and roots and root/leaf nitrate in pistachio seedlings that treated with 150 mM NaCl. The effect of sodium chloride or urea on Na+, K+ and Na+ concentration was significant. The results showed that application of urea under salt stress conditions improved some biochemical indices related to nitrogen metabolism in pistachio seedlings.

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Articles in Press, Accepted Manuscript
Available Online from 30 December 2021
  • Receive Date: 07 March 2019
  • Revise Date: 27 September 2021
  • Accept Date: 28 November 2021
  • First Publish Date: 30 December 2021