Investigation of pmt gene overexpression on morphological, biochemical characteristics and production of tropane alkaloids in Hyoscyamus reticulatus L. hairy roots under the influence of optimal concentration of yeast extract

Document Type : Research Paper

Authors

1 Department of Horticulture, Faculty of Agriculture, Urmia University, Iran.

2 Department of Plant Physiology, Faculty of Pharmacy, University of Barcelona, Av. Joan, XXIII, S/n, 08028, Barcelona, Spain

Abstract

Genetic engineering and elicitors are useful strategies to optimize high level production of secondary metabolites. In this study, hairy roots were produced with inoculated cotyledon explants of Hyoscyamus reticulatus L. with strain A4 of Agrobacterium rhizogenes and strain C58C1 (pRiA4-pBMI) carrying the pmt gene of Agrobacterium tumefaciens. Then, the effect of pmt gene overexpression and optimal concentration of yeast extract (250 mg l-1 after 48 h) were evaluated on morphological, biochemical characteristics and production of tropane alkaloids in hairy roots obtained from two strains. The results of the effect of different Agrobacterium strains with the optimal concentration of yeast extract showed that in hairy roots obtained from C58C1 strain, fresh weight (2.85 times) and dry weight (2.27 times), total phenol (1.74 times), flavonoid (1.76 times) and alkaloid (1.77 times) were higher than the hairy roots obtained from A4 strain. The maximum antioxidant capacity using two methods IC50 (1.62 µg ml-1) and FRAP (14.77 mmol Fe2+ g-1 FW) were observed in hairy roots obtained C58C1 strain. Also, the highest production of hyoscyamine (1.37 times) and scopolamine (1.34 times) was obtained in hairy roots from C58C1 strain. The results showed that the combination of pmt gene overexpression and the optimal concentration of yeast extract could be used as a suitable method to increase the production of phytochemicals, antioxidant capacity, activity of antioxidant enzymes and tropane alkaloids in Hyoscyamus reticulatus L. hairy roots.

Keywords


Articles in Press, Accepted Manuscript
Available Online from 26 December 2021
  • Receive Date: 13 April 2021
  • Revise Date: 29 July 2021
  • Accept Date: 28 September 2021
  • First Publish Date: 26 December 2021