Comparison of fatty acids and hydrocarbons of Milk thistle in different levels of water stress

Document Type : Research Paper

Authors

1 PhD student of Mohaghegh Ardabili University

2 Assistant Professor, Shahid Beheshti University, Faculty of Life Sciences and Biotechnology, Department of biotechnology

3 4- Assistant Professor, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

4 Assistant Professor, Department of Agronomy & Plant Breeding, Faculty of agriculture and natural resources, University of Mohaghegh Ardabili, Ardabil, Iran

Abstract

Water stress is one of the most important factors affecting the metabolic pathways in the plant and thus alters the synthesis and accumulation of natural compounds such as fatty acids and hydrocarbons in medicinal plants. This study aimed to investigate the effect of different levels of water stress (Full irrigation at field capacity, irrigation at 70% of field capacity and irrigation at 40% of field capacity) on fatty acid and hydrocarbon quantities of Milk thistle. Drought stress was applied at flowering stage. Then the oil extract was extracted from the seeds using Soxhlet method and analyzed by GC-MS. Information about the pathway of the reactions of the identified compounds was extracted from bioinformatics databases. The results showed that the yield of fatty acids and hydrocarbons increased with increasing drought stress intensity. The predominant fatty acids identified in Milk thistle oil extract was linoleic acid (15.2-27.4%) and its dominant hydrocarbon was dodecane (12.3-14.1%). The percentage of fatty acids detected under stress was reduced, probably due to their degradation into hydrocarbons to maintain osmotic pressure under dehydration.

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Articles in Press, Accepted Manuscript
Available Online from 03 April 2021
  • Receive Date: 09 February 2020
  • Revise Date: 05 July 2020
  • Accept Date: 28 September 2020
  • First Publish Date: 03 April 2021