In order to study the effect of uniconazole and bio fertilizers on grain yield, chlorophyll fluorescence indices and some physiological traits of wheat under salinity soil conditions, a factorial experiment was conducted based on RCBD with three replications in 2018. Treatments were included soil salinity in four levels (non-application of salinity as control (1.72 dS m−1 and application of 40, 80 and 120 mM salinity in soil or equivalent to 3.68, 7.37 and 11.06 dS m−1, respectively), by NaCl and application of uniconazole and bio fertilizers in seven levels (control or no application of bio fertilizers and uniconazole, application of uniconazole, mycorrhiza fungi, pseudomonas putida, mycorrhiza with pseudomonas, mycorrhiza with uniconazole, both application of mycorrhiza with uniconazole and pseudomonas). Means comparision showed that both application of mycorrhiza with uniconazole and pseudomonas under no salinity condition increased maximum fluorescence (82.17 %), variable fluorescence (287.5 %), quantum yield (65.41 %), chlorophyll index (106.23 %) , leaf nitrogen content (76.66 %) and relative water content of flag leaf (101.76 %) in comparison with no application of bio fertilizers and uniconazole under 120 mM salinity condition. Maximum of minimum fluorescence (F0) and electrical conductivity were obtained at the highest soil salinity level. Also, both application mycorrhiza with pseudomonas and uniconazole increased grain yield per plant about 108.84% in comparison with no application of bio fertilizers at the highest soil salinity level.