Document Type : Research Paper

Authors

• Mino Asadi
• Faranak Hadi
• Seyed Hesamaldin Hejazi
• Farideh Azarbani

Department of Biology, Faculty of Basic Sciences, Lorestan University, Khorramabad, Iran

Abstract

The use of medicinal plant is one of the most common treatments in traditional and modern medicine due to their various potential properties. In this study, phytochemical compounds, antibacterial and anticancer activity of aqueous extract of Allium canadense were evaluated for the first time in the word. Total phenolic and flavonoid contents and functional groups of compounds were assessed using Folin-Sicalto, aluminum chloride colorimetric and FTIR spectroscopy, respectively. Cytotoxicity effect of the different concentration of extract 50, 100, 150 and 200 μg/ml at 24, 48 and 72 hours against MCF-7 breast cancer cell line was determined based on MTT assay. Also, the antibacterial properties of the plant extract were evaluated by disk diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were carried out by broth microdilution method. Phenolic and flavonoid contents of aqueous extract of the plant were118/06±0/02mg/g of dried extract and 90/16±0/03 mg/g quercetin per gram of dry extract, respectively. FTIR spectral analysis also showed the existence of biomolecules containing hydroxyl group and aromatic ring. Treatment of breast cancer cells with different concentrations of Sirmouk extract showed anti-proliferation effects, which had the highest effect at 72 hours with 200μg/ml and at 48 hours with 200 and 150μg/ml. The aqueous extract had more bactericidal effect on gram-negative which inhibited the growth of Pseudomonas aeruginosa more than others. The positive effect of aqueous extract of Sirmouk against cancer cell and bacteria may be due to the presence of different compounds like as phenol and flavonoid in the extract.

Keywords

• Antibacterial
• Cytotoxicity
• phenol content
• flavonoid content
• Allium canadense

Main Subjects

• Biochemistry