Document Type : Research Paper

Authors

• Mojgan Soleimanizaadeh ¹
• Mokhtar Jalali Javaran ²
• Abdolreza Bagheri ³
• Mahdi Behdani ⁴

¹ University of hormozgan

² Department of Biotechnology, Tarbiat Modares University, Tehran, Iran

³ Department of Biotechnology and Plant Breeding, Ferdowsi University of Mashhad, Mashhad, Iran

⁴ Venom & Biotherapeutics Molecules Lab, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran

Abstract

Transient expression through viral vectors has emerged as a preferred method for the production of plant recombinant protein. The short-term production of recombinant protein in this method, compared to time-consuming production of stable transgenic plants along with being cheaper and easier strategy pave the way for commercial production of recombinant proteins. In this research, Anti-Vascular endothelial growth factor (AntiVEGF) protein was produced in Chenopodium album plant using Zucchini yellow mosaic virus (ZYMV) virus-based transient expression method. Target plants were initially infected using a viral vector containing the GFP reporter gene (pZYMVGFP) to ensure the transgene expression. After the appearance of viral symptoms on plant leaves and also confirmation of plant infection by RT-PCR and dot blot methods, target gene cloning was performed in the pZYMVGFP virus vector. In the next step, the target plants were inoculated using a recombinant viral vector (pZYMVAntiVEGF) and the target gene expression was examined by RT-PCR at the transcription level and by Dot blot, Western blot and ELISA tests at translation level. Various molecular analysis confirm the recombinant AntiVEGF expression in this plant. Therefore, ZYMV viral-based transient expression system would be a suitable platform for recombinant proteins production in Chenopodium album plants.

Keywords

• Transient expression platform
• Chenopodium album plant
• Plant bioreactor
• Molecular pharming
• Angiogenesis

Main Subjects

• Biotechnology