Document Type : Research Paper

Authors

• Maryam Rajabi-Farshmi ¹
• Reza Shirzadian-khorramabad ²
• amin Abedi ¹
• Mohammad-hossein Rezadoost ¹

¹ Department of Biotechnology, Faculty of Agricultural Sciences, University of Guilan

² Academic member of biotechnology department, university of Guilan

Abstract

Cysteine is an important precursor for defense compounds in plants. The enzyme cysteine synthase (OASTL; EC 2.5.1.47) catalyzes the final stage of cysteine biosynthesis and need cofactor Pyridoxal-Phosphate) PLP) for its function. The use of bioinformatics tools to find genes with functional domain PALP (Pyridoxal-Phosphate dependent enzyme) in Arabidopsis and rice identified 20 and 19 OASTL-Like genes. Physicochemical, phylogenetic, gene structure, conserved motifs, Cis regulatory elements, post-transcription expression regulation and expression profile of OASTL-Like genes in Arabidopsis and rice were investigated. The identified genes have been categorized into two major clusters and several subgroups. The first cluster consists of 20 members all of which have a related function to cysteine synthase and The second cluster has 19 members. Structure of the intron-exon, the composition and distribution of motifs of those genes were similar confirming the phylogenetic classification indicating the high conservation of genes. Evaluation of promoter region OASTL-Like genes identified 47 types of regulatory elements in Arabidopsis and 48 types in rice. Regulatory elements G-box, ARE and ABRE have the highest frequency. Examination of the mRNA sequence of OASTL-Like family genes in Arabidopsis and rice showed that these genes target 87 and 105 different miRNA molecules, which regulate gene expression at post-transcriptional. The expression profile of OASTL-Like genes indicates the very diverse function of these genes in different developmental stages, in responds to abiotic stresses. The results of this study provide basic and valuable information about OASTL-Like genes in plants.

Keywords

• "
• Bioinformatics
• cysteine synthase
• Database
• gene expression
• promoter "

Main Subjects

• Bioinformatic