نوع مقاله : مقاله پژوهشی
کلیدواژهها
موضوعات
عنوان مقاله English
نویسندگان English
In this study, catecholase activity in tomatoes (Solanum lycopersicum) was investigated under unripe and ripe conditions. The main objective of the study was to investigate the effect of pH, amino acids, and temperature on catecholase activity and the catalytic efficiency of the enzyme under different ripening conditions. Catecholase activity was observed in tomatoes (Solanum lycopersicum) under both unripe and ripe conditions. The optimal pH for catecholase activity varied depending on the type of substrate. Glycine and cysteine amino acids acted as inhibitors, reducing catecholase activity and catalytic efficiency. The catalytic efficiency was higher under ripe conditions. The decrease in Km under ripe conditions indicated a higher affinity of catecholase for catechol, which was confirmed by an increase in maximum velocity (Vmax) of catecholase activity in all three conditions (in the presence and absence of amino acids). At low substrate concentrations, the enzyme had a high affinity and consumed the substrate quickly, while increasing the substrate concentration increased the enzyme's affinity, likely due to conformational changes. The type of inhibition of catecholase by glycine and cysteine was independent of the substrate type and dependent on the inhibitor type, and the ripeness or unripeness of the tomato had no effect on it. C
کلیدواژهها English
مجله پژوهشهای گیاهی (مجله زیست شناسی ایران)(علمی)