نوع مقاله : مقاله پژوهشی
کلیدواژهها
موضوعات
عنوان مقاله English
نویسندگان English
Periwinkle is a highly valuable medicinal herb as it is the only source for the production of the anticancer drugs vinblastine and vincristine. The low synthesis and high cost of the drugs derived from the Periwinkle, along with water scarcity as one of the biggest limiting factors in agriculture, led to this research focusing on the effects of the amino acid tryptophan and drought stress on the changes in secondary metabolites of Periwinkle and the genes associated with the synthesis pathways of vinblastine and vincristine. This study was conducted in a completely randomized design with three factors: tryptophan spraying (0 and 250 ppm), drought stress (40 and 100% field capacity), and time (24, 48, 72, and 168 hours) for evaluating relative expression. The assessment of changes in expression pattern showed the highest relative expression for the Dat and Prx genes in all three conditions at 24 hours and 168 hours compared to the control. The Tdc gene, the 24 and 72-hour treatments after applying tryptophan and the 168-hour tryptophan-drought treatment showed down-regulated in relative expression, while other treatment levels showed an increase in relative expression compared to the control. From a physiological perspective, the highest dry weight of the plant was obtained in the tryptophan spraying treatment. Additionally, drought stress and tryptophan led to an increase in the activity of catalase and peroxidase, as well as the content of phenols and flavonoids, with the highest levels of each observed at concentration of 250 ppm under 40% drought stress of FC.
کلیدواژهها English
مجله پژوهشهای گیاهی (مجله زیست شناسی ایران)(علمی)