بررسی ترکیبات آنتی اکسیدانی و فعالیت ضد باکتریایی عصاره برگ گیاه سرخاب کولی (Phytolacca americana L.) و اثر سمیت آن بر سلول‌های سرطانی ریه

Lung cancer is one of the most common cancers, due to the side effects of chemical treatment methods, plants, which are important sources of anti-cancer compounds, can be a suitable treatment option. Also, with the increasing resistance of bacteria to antibiotics and their side effects, the use of medicinal plants in the treatment of bacterial infections is increasing. Therefore, the aim of this study is to evaluate antioxidant compounds and antibacterial properties of Phytolacca americana leaf extract and its inhibitory effect on lung cancer cells. Leaves were collected from Abbas Abad and extracted. Total phenol, flavonoid, and anthocyanin contents were measured. Also, to determine the cytotoxicity of the extracts, the MTT method was used and the antimicrobial activity of the extracts was determined by disk diffusion methods. The results showed that the extract was rich in phenolic compounds and amount was 16.26±0.227 mg/g dw. The effect of the extract on cell growth inhibition in comparison with the control group was significant and concentration and time depended, as the highest percentage of cell growth inhibition at the concentration of 4 mg/ml in extract was 92.12% during 72 hours. The maximum inhibitory effect of leaf extract on gram positive bacteria Staphylococcus aureus was 14±0.127 mm at of 1 g/ml. Therefore, the results of this study suggest that the leaf extract of P. americana can be an accessible source of antioxidant compounds and can be used as a medicinal plant against lung cancer subject to extensive research due to its high anti-cancer properties.