Iranian Biology Society Journal of Plant Research (Iranian Journal of Biology) 2383-2592 28 2 2015 08 23 Producing of embryogenic callus from zygotic embryo in Dorema ammoniacum D. Producing of embryogenic callus from zygotic embryo in Dorema ammoniacum D. 364 373 589 28214 FA Khadijeh Ghasemian Sonbol Nazeri Abdolkarim Chehregani Rad 0000-0002-8977-9676 Asghar Mirzaie Asl 0000-0001-6590-0753 Journal Article 2012 09 23 Dorema ammoniacum is a native medicinal plant in Iran, which due to ruinous harvesting practices and its natural habitats are destroyed in the country, the species is getting endangered. Somatic embryogenesis is considered as useful method for propagation of plants. In the present study induction of embryogenic callus from zygotic embryo of this plant were investigated using MS and ½ MS basal media and also the different concentrations of NAA and BA hormones. It was observed that MS medium containing 1 mg/l NAA with 0.5 mg/l BA hormones was the best medium for callus induction and maximum number of somatic embryos production. Furthermore, MS basal medium compared to ½ MS basal medium showed a better result, not only in the callus formation but also in the somatic embryogenesis. No embryogenesis was observed after sub-culturing of non-embryogenic calluses in hormone-free MS basal medium. When embryos were transferred to media without growth regulators, whole plantlets were regenerated. Dorema ammoniacum is a native medicinal plant in Iran, which due to ruinous harvesting practices and its natural habitats are destroyed in the country, the species is getting endangered. Somatic embryogenesis is considered as useful method for propagation of plants. In the present study induction of embryogenic callus from zygotic embryo of this plant were investigated using MS and ½ MS basal media and also the different concentrations of NAA and BA hormones. It was observed that MS medium containing 1 mg/l NAA with 0.5 mg/l BA hormones was the best medium for callus induction and maximum number of somatic embryos production. Furthermore, MS basal medium compared to ½ MS basal medium showed a better result, not only in the callus formation but also in the somatic embryogenesis. No embryogenesis was observed after sub-culturing of non-embryogenic calluses in hormone-free MS basal medium. When embryos were transferred to media without growth regulators, whole plantlets were regenerated. https://plant.ijbio.ir/article_589_30bb3825e8f631cc6075c0f87bb4978c.pdf