Iranian Biology Society Journal of Plant Research (Iranian Journal of Biology) 2383-2592 26 3 2013 11 22 Micropropagation of Medicinal Purple Coneflower (Echinacea purpurea L.) Using Cotyledon and Hypocotyl Segments Micropropagation of Medicinal Purple Coneflower (Echinacea purpurea L.) Using Cotyledon and Hypocotyl Segments 311 319 220 2638 FA Journal Article 2010 04 12 Purple coneflower is a herbaceous and perennial flowering plant and described as a traditional valuable species. It has several active compounds including phenolic acids and alkamides. In order to survey of micropropagation condition of this plant, an experiment was conducted as a factorial arrangment in completely randomized design with three replications. The factors were including explant type (hypocotyl and cotyledon), BAP hormone (0, 0.2, 0.4, 1.2 and 2.4 mg l^-1) and NAA hormone (0, 0.05, 0.1, 0.3 and 0.6 mg l^-1). Analysis of variance revealed significant differences among some studied treatments for callus induction and regeneration in explants. Results of means comparison for triple interaction among factors indicated that the highest percentage of callus induction occurred on a MS medium containing 0.2 mg l-1 BAP and 0 mg l^-1 NAA (97%) in cotyledon and 0.2 mg l^-1 BAP and 0.6 mg l^-1 NAA (91%) in hypocotyl explant. Moreover, no significant differences observed between two explants. Combination of 0.4 mg l^-1 BAP and 0 mg l^-1 NAA) has the most effective, providing the highest frequency of shoot regeneration (31.5%) and (32.5%) in cotyledon and hypocotyl explants respectively. Purple coneflower is a herbaceous and perennial flowering plant and described as a traditional valuable species. It has several active compounds including phenolic acids and alkamides. In order to survey of micropropagation condition of this plant, an experiment was conducted as a factorial arrangment in completely randomized design with three replications. The factors were including explant type (hypocotyl and cotyledon), BAP hormone (0, 0.2, 0.4, 1.2 and 2.4 mg l^-1) and NAA hormone (0, 0.05, 0.1, 0.3 and 0.6 mg l^-1). Analysis of variance revealed significant differences among some studied treatments for callus induction and regeneration in explants. Results of means comparison for triple interaction among factors indicated that the highest percentage of callus induction occurred on a MS medium containing 0.2 mg l-1 BAP and 0 mg l^-1 NAA (97%) in cotyledon and 0.2 mg l^-1 BAP and 0.6 mg l^-1 NAA (91%) in hypocotyl explant. Moreover, no significant differences observed between two explants. Combination of 0.4 mg l^-1 BAP and 0 mg l^-1 NAA) has the most effective, providing the highest frequency of shoot regeneration (31.5%) and (32.5%) in cotyledon and hypocotyl explants respectively. https://plant.ijbio.ir/article_220_ec8ce6abb3e952a85b8551ba726a1227.pdf