Iranian Biology Society Journal of Plant Research (Iranian Journal of Biology) 2383-2592 31 2 2018 08 23 Improvement of culture media and PGRs in Pistacia vera cv. Ghazvini micropropagation Improvement of culture media and PGRs in Pistacia vera cv. Ghazvini micropropagation 383 395 1151 FA Esmaeel Nezami Department of Plant Breeding, Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Karaj I.R. of Iran. Journal Article 2016 03 01 Pistacia vera cv. Ghazvini rootstock is considered as one of the important pistachio rootstocks in tolerance to salinity, calcareous and resistant to drought. In order to providing a reliable protocol for micro-propagation of this rootstock, the efficiency of modified GNH media (GNH-A and GNH-B) compared to MS, WPM, DKW or GNH media was studied. Moreover, the effect of different concentration of BAP (1.1, 1.8 or 3.6 mg/l) and L-glutamine (0.0, 400, 800 or 1600 mg/l) to improve shoot-let production rate with the lowest physiological disorders was survived. Results revealed that the greatest buds and shoots number per explants (34.46 ± 3.16 and 4.23 ± 0.46, respectively) were attained significantly on GNH-A medium compared to the other media studied. Integration of BAP at different concentrations, affected significantly all the studied growth traits, producing the highest shoot number (6.66 ± 0.48) when GNH-A was supplemented with 3.6 mg/l BAP. Furthermore, application of exogenous 800 mg/l L-glutamine in GNH-A medium improved significantly both shoots height and fresh weight (1.20± 0.06cm and 0.31±0.02g, respectively), in comparison with control. In rooting stage, the incorporation of NAA into media significantly increased rooting percentage compared to IBA. Finally, we strongly believe that these results will open new insights for researchers towards efficient culture media improvements for economically important woody plants, Pistacia vera species, in particular. Pistacia vera cv. Ghazvini rootstock is considered as one of the important pistachio rootstocks in tolerance to salinity, calcareous and resistant to drought. In order to providing a reliable protocol for micro-propagation of this rootstock, the efficiency of modified GNH media (GNH-A and GNH-B) compared to MS, WPM, DKW or GNH media was studied. Moreover, the effect of different concentration of BAP (1.1, 1.8 or 3.6 mg/l) and L-glutamine (0.0, 400, 800 or 1600 mg/l) to improve shoot-let production rate with the lowest physiological disorders was survived. Results revealed that the greatest buds and shoots number per explants (34.46 ± 3.16 and 4.23 ± 0.46, respectively) were attained significantly on GNH-A medium compared to the other media studied. Integration of BAP at different concentrations, affected significantly all the studied growth traits, producing the highest shoot number (6.66 ± 0.48) when GNH-A was supplemented with 3.6 mg/l BAP. Furthermore, application of exogenous 800 mg/l L-glutamine in GNH-A medium improved significantly both shoots height and fresh weight (1.20± 0.06cm and 0.31±0.02g, respectively), in comparison with control. In rooting stage, the incorporation of NAA into media significantly increased rooting percentage compared to IBA. Finally, we strongly believe that these results will open new insights for researchers towards efficient culture media improvements for economically important woody plants, Pistacia vera species, in particular. https://plant.ijbio.ir/article_1151_f197002b9a0853eca5e046d9ca4663d5.pdf