Iranian Biology Society Journal of Plant Research (Iranian Journal of Biology) 2383-2592 31 1 2018 04 21 Combine effect of temperature and photoperiod on growth and biomass of green algae Scenedesmus quadricauda Combine effect of temperature and photoperiod on growth and biomass of green algae Scenedesmus quadricauda 145 155 1133 FA Journal Article 2015 09 30 Temperature and photoperiod affect on the growth and biomass of microalgae. In this study, combined effects of different temperatures (20, 25 and 30°C) and different photoperiods (8L:16D, 12L:12D and 16L:8D) on the density, specific growth rate (SGR), doubling time (Dt) and biomass of freshwater green microalgae Scenedesmus quadricauda were investigated. The experiment was carried out as completely randomized design in Bold Basal,s Medium (BBM) for 16 days. Results showed that density, specific growth rate, doubling time and biomass are significant differences among treatments (P< 0.05). The maximum density (2.11×107 cell/ml), specific growth rate (0.24 /day), biomass (0.30 mg/ml) and the minimum doubling time (2.87 day) were obtained at 16L:8D photoperiod and 30 °C. The minimum density (8.18×106 cell/ml), minimum SGR (0.178 /day), minimum dry biomass (0.094 mg/ml) and maximum Dt (3.897 day) were at 8L:16D light/dark photoperiod and 20 °C, respectively. Based on obtained results, it is concluded that the increasing of light hours as photoperiod in combination with higher temperature could be make better performance for S. quadricauda in mass culture. Temperature and photoperiod affect on the growth and biomass of microalgae. In this study, combined effects of different temperatures (20, 25 and 30°C) and different photoperiods (8L:16D, 12L:12D and 16L:8D) on the density, specific growth rate (SGR), doubling time (Dt) and biomass of freshwater green microalgae Scenedesmus quadricauda were investigated. The experiment was carried out as completely randomized design in Bold Basal,s Medium (BBM) for 16 days. Results showed that density, specific growth rate, doubling time and biomass are significant differences among treatments (P< 0.05). The maximum density (2.11×107 cell/ml), specific growth rate (0.24 /day), biomass (0.30 mg/ml) and the minimum doubling time (2.87 day) were obtained at 16L:8D photoperiod and 30 °C. The minimum density (8.18×106 cell/ml), minimum SGR (0.178 /day), minimum dry biomass (0.094 mg/ml) and maximum Dt (3.897 day) were at 8L:16D light/dark photoperiod and 20 °C, respectively. Based on obtained results, it is concluded that the increasing of light hours as photoperiod in combination with higher temperature could be make better performance for S. quadricauda in mass culture. https://plant.ijbio.ir/article_1133_fd06b8ea02fe5b1c2496fe1700e9d16c.pdf