Iranian Biology Society Journal of Plant Research (Iranian Journal of Biology) 2383-2592 29 2 2016 08 22 The in vitro antibacterial activity of different organs hydroalcoholic extract of Dendrostellera lesserti The in vitro antibacterial activity of different organs hydroalcoholic extract of Dendrostellera lesserti 407 421 1073 FA Mustafa Alamhulu , Department of Agricultural Biotechnology, Bu-Ali Sina University, Sonbol Nazeri Department of Agricultural Biotechnology, Bu-Ali Sina University Journal Article 2014 06 23 Resistance of many diseases pathogenic bacteria against synthetic drugs and their side effects ,besides high expense of chemical drugs directed the attention to scientists toward natural and herbal medicines usage. The purpose of this study is to investigate the antibacterial properties of root, leaf and stem hydroalcoholic extract of Dendrostellera lesserti against some human pathogenic bacteria. Dendrostellera lesserti was collected from Hamedan province in 2013. After plant identification, the extracts were prepared using maceration method. The eight positive and negative gram human pathogenic bacteria were tested. For‌‌ antibacterial‌ properties investigation, agar well diffusion, MIC (serial dilution method) and MBC methods were used. The amount of total phenol was measured by Folin-ciocalteu. The results was statistically analysis using sas 9.2 in factorial with completely randomized design in three replications. Though, the highest growth inhibition zone (18±1.00 mm) in Bacillus subtillis culture in presence of root hydroalcoholic extract, the stem hydroalcoholic extract showed more inhibitory and bactericidal effect on the most bacterial cultures. The amount of total phenol in the root, stem and leaf was measured as, 111.8± 2.69, 79.47±0.55 and 69.16±3.29 (mgGAE/g), respectively. According to the results, the root, leaf and stem hydroalcoholic extracts of Dendrostellera lesserti contain different amount of phenol and plant contains compounds with antibacterial properties. This research can be used as a base in antimicrobial compounds study of this plant. Resistance of many diseases pathogenic bacteria against synthetic drugs and their side effects ,besides high expense of chemical drugs directed the attention to scientists toward natural and herbal medicines usage. The purpose of this study is to investigate the antibacterial properties of root, leaf and stem hydroalcoholic extract of Dendrostellera lesserti against some human pathogenic bacteria. Dendrostellera lesserti was collected from Hamedan province in 2013. After plant identification, the extracts were prepared using maceration method. The eight positive and negative gram human pathogenic bacteria were tested. For‌‌ antibacterial‌ properties investigation, agar well diffusion, MIC (serial dilution method) and MBC methods were used. The amount of total phenol was measured by Folin-ciocalteu. The results was statistically analysis using sas 9.2 in factorial with completely randomized design in three replications. Though, the highest growth inhibition zone (18±1.00 mm) in Bacillus subtillis culture in presence of root hydroalcoholic extract, the stem hydroalcoholic extract showed more inhibitory and bactericidal effect on the most bacterial cultures. The amount of total phenol in the root, stem and leaf was measured as, 111.8± 2.69, 79.47±0.55 and 69.16±3.29 (mgGAE/g), respectively. According to the results, the root, leaf and stem hydroalcoholic extracts of Dendrostellera lesserti contain different amount of phenol and plant contains compounds with antibacterial properties. This research can be used as a base in antimicrobial compounds study of this plant. https://plant.ijbio.ir/article_1073_390e982518a50e280d8e2b535462ec1f.pdf