TY - JOUR ID - 968 TI - Investigation of callus induction and indirect shootregeneration in Chelidonium majus L. affected by plant growth regulators JO - Journal of Plant Research (Iranian Journal of Biology) JA - JPR LA - en SN - 2383-2592 AU - Esfandiar, Akram AD - Y1 - 2017 PY - 2017 VL - 30 IS - 3 SP - 488 EP - 497 KW - Regeneration KW - Callus KW - tissue culture KW - Celandine DO - N2 - Celandine (Chelidonium majus L.),a member of Papaveraceae family, is a valuable medicinal plant. Becausemany active substances have medicinal properties that make it great. Inorder to study the effect of hormones 2,4-dichlorophenoxy acetic acid (2,4-D)in five levels (0, 0.5, 1, 1.5 and 2 mg/l) and with hormone benzyladenine (BA) at four levels (0.25, 0.5, 1 and1.5 mg/l)for callus, explant using stems, leaves and seeds were evaluated. To assess theregeneration of combined hormonal BAP at three levels (1, 2 and 3 mg/l)of 2,4-D hormone on two levels (0.1 and 0.5 mg/l) was used. This was a factorial experiment based on randomized complete designwith three replications. The results showed that the best environment for theinduction of callus induction on MS medium containing 1 mg/l 2,4-D and 0.5mg/l BA %69.44 % rate of 2 mg/l 2,4-D and 1.5 mg/l BA %63.88 % respectively. The best medium for callus on MS medium containing 2 mg/l 2,4-D wetfresh weight. Best Celandine explants to form callus weight and stem explants. Most regeneration and leaf production in MS medium containing 2 mg/l BAP and 0.5 mg/l 2,4-D was observed but did not shoot. UR - https://plant.ijbio.ir/article_968.html L1 - https://plant.ijbio.ir/article_968_a6955b6ad6bd6ef51d637e17de37c7cc.pdf ER -