TY - JOUR ID - 1568 TI - Evaluation of the effect of iron sulfate on growth and some biochemical parameters of garlic plantlets under in vitro culture condition JO - Journal of Plant Research (Iranian Journal of Biology) JA - JPR LA - en SN - 2383-2592 AU - Fathi Rezaei, Parisa AU - Mohammadnezhad, Marya AU - Aghaee, Ahmad AD - Department of Biology, Faculty of Science, University Of Maragheh AD - Department of Biology, Faculty of Science, University of Maragheh, Maragheh, Iran AD - Biology department, Faculty of Science, University of Maragheh, Prof. Ghannadi Boulevard, Mother Square, Maragheh, Iran Y1 - 2020 PY - 2020 VL - 32 IS - 4 SP - 898 EP - 909 KW - Allicin KW - cysteine KW - fresh weight KW - garlic KW - protein DO - N2 - Allicin as the best-known active compound of garlic (Allium sativum) has a vast variety of biological effects. The effect of iron sulfate was evaluated on allicin, cysteine, growth and protein contents of Allium sativum explants. Garlic bulbs were surface-sterilized and cultured on Murashige and Skoog (MS) medium for 1 month then transferred to the medium supplemented with different concentrations of iron sulfate for 10 and 20 day periods. At the end, shoot and root samples were gathered and fresh weighted and length of plantlets measured. Allicin content was determined by HPLC method, cysteine and total protein contents were determined by spectrophotometry. The highest amount of allicin and protein was observed for 11.12 mg/L treatment of root and shoot at both endpoints. The maximum content of allicin and protein were determined respectively on the root (16.62 mM/g FW) and shoot (8 mg/g FW) of 11.12 mg/L-treated explants after 20 days. On 5.56 mg/L-treated explants, a significant increase of fresh weight and length of root and cysteine content were observed in comparison with other treatments. In addition, on the shoot of 1.39 mg/L-treated explants, a significant difference in fresh weight and length were measured compared with the other treatments. Because of the important role of allicin on medicinal features of Allium sativum, probably iron sulfate could be a good elicitor for the elevation of allicin content of garlic explants. UR - https://plant.ijbio.ir/article_1568.html L1 - https://plant.ijbio.ir/article_1568_1ac58d31c7767ae83c52c75c25d221ab.pdf ER -